Collecting Fractions Automatically (Autopurification)
Setting up Autopurification

Before you can collect fractions automatically, you need to take the necessary preparative steps in Chromeleon, using the corresponding files from the Additional_Software_Autopurification CD. First, select the appropriate directory for your installation, e.g., APS_2222_UV_MS.

Windows Explorer

Copy the Dionex_Purification_Parameters.GEN file from the Drivers subdirectory to the Chromel/bin directory.

 Tip:

Usually, a Dionex Service Representative or an authorized Dionex distributor copies the file. (Make sure that you do not move any chromatographic files in the Windows Explorer. The reason is that, except for the visible results, processes are performed below the surface.)

Server Configuration

Install the devices in the Server Configuration program. Usually, you have to install the single devices manually. However, for autopurification, Chromeleon provides several predefined device configurations on the CD that is shipped with the product. For example:

APS_2222_UV_MS.CFG (in the cfg file directory)

Select Import on the File menu and import the configuration.

PGM Files

In the PGM File, define how the single fractions are to be collected. The Programs directory provides different programs as Backup Files. Select Restore on the File menu and specify the location to which you want to restore the program(s).

Or else, you may create a program in the Program Wizard (see The Control Program  The Program Wizard). For more information, refer to  Collecting Fractions.

Control Panels

Install the predefined control panels from the Panels directory on the CD to an appropriate location, e.g., the Dionex Templates>Panels directory in the local datasource of the Chromeleon Browser. For example, create an Autopurification folder in this directory.

Reports

In the same way, you may copy the report templates from the Reports directory on the CD to an appropriate location, e.g., the Dionex Templates>Reports directory in the local datasource of the Chromeleon Browser. For example, create an Autopurification folder in this directory.

Demo Data

In addition, demo data backup files are provided in the Demo_data directory. Copy the data to an appropriate location, if required.

For an overview of how to perform Autopurification, refer to  Collecting Fractions Automatically (Autopurification).